Recombinant Estrogen Receptor alpha anticorps (AA 495-595)

N° du produit ABIN7477471

Cet anticorps anti-Estrogen Receptor alpha est un anticorps Lapin Monoclonal détectant Estrogen Receptor alpha dans IHC. Adapté pour Humain.

Aperçu rapide pour Recombinant Estrogen Receptor alpha anticorps (AA 495-595) (ABIN7477471)

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Monoclonal

Conjugué: Cet anticorp Estrogen Receptor alpha est non-conjugé

Application: Immunohistochemistry (IHC)

Clone: MSVA-564R

Détail du produit anti-Estrogen Receptor alpha anticorps

Épitope: AA 495-595

Immunogène: Recombinant fragment (around aa495-595) of human ER alpha protein (exact sequence is proprietary)

Isotype: IgG

Information d'application

Indications d'application: IHC 1:100-1:200

Commentaires:

Positive Control: Uterine cervix: virtually all squamous and endocervical cells must show a moderate to strong nuclear staining. Tonsil: Scattered follicular dendritic cells in germinal centers and some squamous epithelial cells in tonsil crypts must show an at least weak nuclear ER immunostaining.

Negative Control: Tonsil: B-cells in mantle zones and within germinal centers must be negative.

Protocole: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Stockage

Format: Liquid

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Détails sur Estrogen Receptor alpha

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Autre désignation: Estrogen Receptor

Sujet: Estrogen Receptor alpha delta 4*5,6,7*/654 isoform,Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1,Estrogen Receptor antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly